주요연구분야
1. DNA and RNA measurement standards
Accurate nucleic acid quantification is essential for the development of molecular diagnoses and therapeutic research. Our laboratory focuses on the developing accurate measurement methods to achieve absolute quantification of specific nucleic acid targets. By using these methods, we develop reference materials and certified reference materials related to molecular testing.
- Quantitative gene measurement (digital PCR, real-time PCR, LC-MS, imaging-based counting)
- Qualitative characterization (high-throughput sequencing, size distribution)
- Molecular diagnosis (cancer biomarkers, pathogen gene detection)
2. Biopharmaceuticals measurements
We also apply our methods to ensure accurate analysis and measurement for biopharmaceuticals such as gene and cell therapy products.
3. DNA fragmentation as an indicative for cytotoxicity
Regulated cell death plays an essential role in various biological processes, leading to the development of a number of methods to detect and quantitatively measure cells exhibiting decreased viability due to either apoptosis or necrosis. When cytotoxicity is induced, human cell lines exhibit specific features, including dampened cell proliferation and lost plasma membrane asymmetry, presenting distinct sensitivity. Our team is developing a set of novel digital PCR (dPCR) assays to quantitatively measure the degree of cytotoxicity.
주요활동(최근5년)
Jina Kim, Joohon Sung, Kyudong Han, Wooseok Lee, Seyoung Mun, Jooyeon Lee, Kunhyung Bahk, Inchul Yang, Young-Kyung Bae, Changhoon Kim, Jong-Il Kim and Jeong-Sun Seo. A High Quality Asian Genome Assembly Identifies Features of Common Missing Regions (2020) Genes 2020, 11(11), 1350; https://doi.org/10.3390/genes11111350
The dMIQE Group, Jim F Huggett. The Digital MIQE Guidelines Update: Minimum Information for Publication of Quantitative Digital PCR Experiments for 2020 (2020) Clinical Chemistry vol 66; 8 (1012-1029)
Kim DY, Lee SS, Bae Y.-K. Colorectal cancer cells differentially impact migration and miRNA expression in endothelial cells. (2019) Onc. Letters. Nov 5 2019 vol 18; 6 (6361-6370)
Lee SS, Won JH, Lim GJ, Han J, Lee JY, Cho KO* and Bae Y.-K*. A novel population of extracellular vesicles promotes cell proliferation (2019) Cell Communication and Signaling. *co-corresponding. Aug 15; 95
Won JH, Kim GW, Kim JY, Cho DK, Lee SS, Kwon BK, Bae Y.-K, and Cho KO. Cleavage of extracellular Wingless (Wg) by Sol narae (Sona) produces Wg-CTD, a novel form of active Wg. (2019) Cell Death and Differentiation. 2019 Aug; 10(8): 564.
Han J, Lee JY, and Bae Y.-K. Application of digital PCR for assessing DNA fragmentation in cytotoxicity response (2019) Biochim Biophys Acta Gen Subj. Aug;1863(8):1235-1242.
Kwon HJ, Jeong JS, Bae, Y.-K., Choi K and Yang I. Stable Isotope Labeled DNA: A New Strategy for the Quantification of Total DNA Using Liquid Chromatography–Mass Spectrometry. (2019) Anal. Chem. 91,6,3936-3943
Tsogtbaatar O,Won JH, Kim KW, Han JH, Bae Y.-K*, and Cho KO*. An ADAMTS Sol narae is required for cell survival in Drosophila (2019) Scientific Reports. Feb 4;9(1):1270. *co-corresponding
Alexandra S. Whale, Gerwyn M. Jones, Jernej Pavšič, Tanja Dreo, Nicholas Redshaw, Sema Akyurek, Muslum Akgoz, Carla Divieto, Maria PaolaSassi, Hua-Jun He, Kenneth D. Cole, Young-Kyung Bae, Sang-Ryoul Park, Liesbet Deprez, Philippe Corbisier, Sonia Garrigou, Valérie Taly, Raquel Larios, Simon Cowen, Denise M. O'Sullivan, Claire A. Bushell, Heidi Goenaga-Infante, Carole A. Foy, Alison J. Woolford, Helen Parkes, Jim F. Huggett, Alison S. Devonshire. Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine. (2018) Clinical Chemistry. 2017.285478
Bae Y.-K., Macabenta F, Curtis HL, Stathopoulos A Comparative analysis of gene expression profiles for several migrating cell types identifies cell migration regulators. (2017) Mech Dev. Dec;148:40-55.
Stepanik, V., Dunipace, L., Bae, Y.-K., Macabenta, F., Sun, J., Trisnadi, N., Stathopoulos, A. (2016) The migrations of Drosophila muscle founders and primordial germ cells are interdependent. Development, Sep 1;143(17):3206-15.
